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robosep automated cell isolation system  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc robosep automated cell isolation system
    Robosep Automated Cell Isolation System, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/automated+cell+isolator+(robosep/robosep+automated+cell+separator/pm34077868-47-23-28
    Average 90 stars, based on 1 article reviews
    robosep automated cell isolation system - by Bioz Stars, 2026-10
    90/100 stars

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    Isolation:

    Article Title: M2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway
    Article Snippet: In brief, mononuclear cells were isolated from whole blood using density gradient centrifugation (Lymphoprep; Axis-Shield). .. After this, monocytes were isolated using an automated cell isolator (RoboSep; StemCell Technologies, Inc.) and CD14 selection kit (EasySep Human Buffy Coat; StemCell Technologies, Inc.). .. The monocytes were differentiated in AIM-V media (Invitrogen) supplemented with the following specific cytokines: M1 macrophages, 5 ng/ml recombinant human GM-CSF (Sigma-Aldrich) for 7 d followed by 5 ng/ml recombinant human GM-CSF, 5 ng/ml recombinant human IFN-γ (R&D Systems), and 100 ng/ml lipopolysaccharide (Sigma-Aldrich) for 2 d; M2 macrophages, 20 ng/ml recombinant human M-CSF and 12.5 ng/ml recombinant human IL-4 (both obtained from R&D systems) for 9 d; and resting macrophages were generated by culturing for 9 d without cytokines.

    Selection:

    Article Title: M2-like macrophages are responsible for collagen degradation through a mannose receptor–mediated pathway
    Article Snippet: In brief, mononuclear cells were isolated from whole blood using density gradient centrifugation (Lymphoprep; Axis-Shield). .. After this, monocytes were isolated using an automated cell isolator (RoboSep; StemCell Technologies, Inc.) and CD14 selection kit (EasySep Human Buffy Coat; StemCell Technologies, Inc.). .. The monocytes were differentiated in AIM-V media (Invitrogen) supplemented with the following specific cytokines: M1 macrophages, 5 ng/ml recombinant human GM-CSF (Sigma-Aldrich) for 7 d followed by 5 ng/ml recombinant human GM-CSF, 5 ng/ml recombinant human IFN-γ (R&D Systems), and 100 ng/ml lipopolysaccharide (Sigma-Aldrich) for 2 d; M2 macrophages, 20 ng/ml recombinant human M-CSF and 12.5 ng/ml recombinant human IL-4 (both obtained from R&D systems) for 9 d; and resting macrophages were generated by culturing for 9 d without cytokines.



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